茶叶有效成分抗肿瘤侵袭转移作用与相关机制探讨

Effect of EGCG on invasion of breast cancer cells and its possible mechanism

目的观察茶叶的有效成分表没食子儿茶素没食子酸酯[(-)-epigallocatechin-3-gallate(EGCG)]对人乳腺癌高转移细胞侵袭转移的影响作用及探讨其相关机制。方法用免疫细胞化学法检测MUC1的表达,以人工重组基底膜侵袭小室(Transwell)及明胶酶谱法(PAGE)观察其对人乳腺癌高转移细胞株MDA-MB-231细胞侵袭转移能力的影响。结果茶叶有效成分EGCG能降低MDA-MB-231细胞MUC1表达,明显抑制MDA-MB-231细胞侵袭基底膜的能力和Ⅳ型胶原酶的产生。结论茶叶有效成分EGCG具有明显的抗癌侵袭转移作用,此与降低MUC1的表达相关。

AIM： To stud, the effects of （ - ） - epigallocatechin - 3 - gallate （EGCG）, a tea extract, on the invasion and metastasis of breast cancer cell line MDA - MB - 231 and the possible mechanisms in vitro. METHODS： The expression of MUCI in breast cancer cells treated with or without EGCG was detected by immunohistochemistry. The effect of EGCG on invasion of MDA - MB - 231 cells was evaluated using Transwell chambers attached with pnlycarbonate filters and reconstituted basement memhrane （Matrigel）. Gelatin zymography was performed to detect the secretion of collagenase-Ⅳ . RESULTS： EGCG reduced the expression of MUCI, significantly suppressed the invasion of tumor cells to basement membrane and reduced the secretion of collagenase - Ⅳ. CONCLUSION： In vitro, EGCG may suppress invasion, metastasis, and eollagenase- Ⅳ secretion in MDA - MB - 231 cells by inhibiting the production of MUCI.