从G-CSF动员的外周血细胞悬液培养的成纤维细胞样细胞的特性分析

Characters of the fibroblast-like cells cultured from the mobilized peripheral blood cells

目的研究从G-CSF动员的外周血细胞(PBC)悬液培养的成纤维细胞样(F-L)细胞的特性。方法取PBC中贴壁细胞分4组培养①RPMI-1640组;②L-DMEM组;③粒细胞集落刺激因子(G-CSF)组;④白细胞介素-3(IL-3)组。流式细胞仪分析各组培养的F-L细胞特性。结果培养2-3周后,4组均能收获到F-L细胞,细胞贴壁生长,但不融合,不能连续传代培养,③④组细胞数量明显多于①②组,4组F-L细胞表型相似CD33+、CD11c+、CD64+、CD14+、CD45+、HLA-DR+、CD86+、CD34-、CD38-、CD3-、CD19-、CD56-、CD29-、CD44-、CD105-;与单核细胞(PB-M)表型差异仅CD38表达不同而与间质干细胞(MSC)或树突状细胞(DC)表型明显不同。结论从PBC培养的F-L细胞为巨噬细胞,不是MSC或DC;G-CSF、IL-3能提高F-L细胞培养数量,不改变PB-M向巨噬细胞分化的分化方向。

AIM： To explore the characters of fibroblast - like （F- 1,） cells cultured from granunoeyte elony stimunating factor （G- CSF） - mobilized peripheral blood cell （PBC） harvests. METHODS： The adherent cells in the PBC harvests were eultured for 2 week in the mediums of RPMI - 1640/L- DMEM/G - CSF or interleukin - 3 （IL- 3） plus RPMI - 1640, the cultured F- L cells were analyzed by flow cytometry （FC）. RESULTS： The adherent non- confluent F- L cells obtained from the four groups were similar in their phenotypes： CD33^＋ , CD11c^＋ , CD64^＋ , CD14^＋ , CD45^＋ , HLA - DR^＋ , CD86^＋ , CD34^- , CD38^- , CD3^- , CD19^- , CD56^- , CD29^- , CD44^- , CD105^- . The F - L cells are similar to monocytes except CD38^- and were distinct from dendritic cells （DC） or mesenehymal stem cells （MSC）. CONCLUSION： The cultured F- L cells are maerophages rather than DC or MSC. G- CSF, rhIL- 3 enhances their numbers.