摘要
目的研究从G-CSF动员的外周血细胞(PBC)悬液培养的成纤维细胞样(F-L)细胞的特性。方法取PBC中贴壁细胞分4组培养①RPMI-1640组;②L-DMEM组;③粒细胞集落刺激因子(G-CSF)组;④白细胞介素-3(IL-3)组。流式细胞仪分析各组培养的F-L细胞特性。结果培养2-3周后,4组均能收获到F-L细胞,细胞贴壁生长,但不融合,不能连续传代培养,③④组细胞数量明显多于①②组,4组F-L细胞表型相似CD33+、CD11c+、CD64+、CD14+、CD45+、HLA-DR+、CD86+、CD34-、CD38-、CD3-、CD19-、CD56-、CD29-、CD44-、CD105-;与单核细胞(PB-M)表型差异仅CD38表达不同而与间质干细胞(MSC)或树突状细胞(DC)表型明显不同。结论从PBC培养的F-L细胞为巨噬细胞,不是MSC或DC;G-CSF、IL-3能提高F-L细胞培养数量,不改变PB-M向巨噬细胞分化的分化方向。
AIM: To explore the characters of fibroblast - like (F- 1,) cells cultured from granunoeyte elony stimunating factor (G- CSF) - mobilized peripheral blood cell (PBC) harvests. METHODS: The adherent cells in the PBC harvests were eultured for 2 week in the mediums of RPMI - 1640/L- DMEM/G - CSF or interleukin - 3 (IL- 3) plus RPMI - 1640, the cultured F- L cells were analyzed by flow cytometry (FC). RESULTS: The adherent non- confluent F- L cells obtained from the four groups were similar in their phenotypes: CD33^+ , CD11c^+ , CD64^+ , CD14^+ , CD45^+ , HLA - DR^+ , CD86^+ , CD34^- , CD38^- , CD3^- , CD19^- , CD56^- , CD29^- , CD44^- , CD105^- . The F - L cells are similar to monocytes except CD38^- and were distinct from dendritic cells (DC) or mesenehymal stem cells (MSC). CONCLUSION: The cultured F- L cells are maerophages rather than DC or MSC. G- CSF, rhIL- 3 enhances their numbers.
出处
《中国病理生理杂志》
CAS
CSCD
北大核心
2005年第11期2264-2267,共4页
Chinese Journal of Pathophysiology
基金
深圳市科技攻关项目(No.199904003)
关键词
树突细胞
间质干细胞
单核细胞
成纤维样细胞
巨噬细胞
Dendritic cells
Mesenchymal stem cells
Monocytes
Fibroblast- like cells
Macrophages