子宫肌瘤致病相关基因的筛选与克隆

Screening and Cloning of Genes Related to Uterine Leiomyoma

【目的】 研究子宫肌瘤与正常子宫肌组织中的基因表达差异，筛选及克隆子宫肌瘤的致病相关基因。【方法】 应用荧光标记的mRNA差异显示技术，比较11例子宫肌瘤及其正常子宫肌组织基因表达的差异，对获得差异片段进行克隆、测序及同源性分析，并对其中5条差异片段在子宫肌瘤及正常子宫肌组织中的表达情况进行RT-PCR分析。【结果】 差异片段NO.3，5，11，12在子宫肌瘤正常及异常组织中的表达均存在差异。其中NO.3差异片段在8例子宫肌瘤组织中有表达，而相应的正常组织中仅2例有表达。NO.5，11，12差异片段在子宫肌瘤中的表达水平高于正常子宫肌组织中的表达水平。【结论】 在子宫肌瘤的发生和发展过程中存在多个基因的表达异常，其中ulap8基因有可能在子宫肌瘤组织中呈特异性的表达，ulap14，ulap25，ulap26基因在子宫肌瘤组织中存在表达差异，推测这些基因有可能与子宫肌瘤的发病有关。

[Objective] To study the alterations of gene expression between uterine leiomyoma tissues and normal myometrium uterine tissues; to screen and clone the genes related to uterine leiomyoma.[ Methods ] To compare different genes expressed in 11 uterine leiomyoma tissues and normal myometrium uterine tissues by fluorescent differential display reverse transcription polymerase chain reaction （FDD-RTPCR）. Differentially expressed eDNA fragments were cloned, sequenced, and analyzed in origin. And five of differentially expressed bands were analyzed by RT-PCR. [Results] The expression of NO. 3, 5, 11, and 12 eDNA fragments in uterine leiomyoma were differential. NO.3 eDNA fragment was expressed in eight of uterine leiomyoma tissue （8/10）, but in two of corresponding normal myometrium tissue. NO. 5, 11, and 12 eDNA fragments were expressed higher in uterine leiomyoma tissue than matched myometrium. [Conclusion] During occurrence and development of uterine leiomyoma, expression of more than one gene altered in uterine myometrium, ulap8 gene may be expressed specially in uterine leiomyoma tissue. ulap14, ulap25, and ulap26 genes were expressed higher in uterine leiomyoma tissue than matched myometrium, it may be related to the development of uterine leiomyoma.