膜型基质金属蛋白酶-1在肝癌组织中的表达及意义

Expression of Membrane Type-1 Matrix Metalloproteinase in Hepatocellular Carcinoma

【目的】比较膜型基质金属蛋白酶-1(membrane type-1 matrix metalloproteinase，MT1-MMP)在大肝癌(瘤体直径＞3cm)及小肝癌(单肿瘤结节且瘤体直径≤3cm)组织中的表达差异，并探讨其在肝癌肿瘤细胞浸润、转移过程中的作用。【方法】应用半定量RT-PCR方法检测MT1-MMP mRNA在22例大肝癌及12例小肝癌组织中的表达；应用免疫组化方法检测MT1-MMP蛋白在53例大肝癌及40例小肝癌组织中的表达，并分析MT1-MMP蛋白表达水平与肝癌浸润、转移之间的关系。【结果】MT1-MMP蛋白在大、小肝癌组织中的表达差异显著(P=0.007)，但其mRNA水平的差异无统计学意义(P=0.693)；MT1-MMP蛋白表达水平与肝癌浸润及转移之间均呈正相关关系(P=0.021；P=0.001)。【结论】调控MT1-MMP蛋白在肝癌组织中异常表达的关键环节可能是在转录后水平进行，大肝癌肿瘤细胞的侵袭能力较小肝癌强与其高表达MT1-MMP蛋白有关。

[Objective] The different expression pattern of membranetype-1 matrix metalloproteinase （MT1- MMP） was detected between advanced HCC （the diameter〉3 cm） and SHCC （simple tumor nodule, the diameter ≤ 3 cm）. The role of MT1-MMP in tumor invasion and metastasis was discussed. [Methods] The expression of MT1-MMP mRNA in 22 cases of advanced HCC and 12 cases of SHCC was detected by semi-quantitative RT-PCR. The expression of MT1-MMP protein in 53 cases of advanced HCC and 40 cases of SHCC were detected by immunohistochemistry. Moreover, the relationship between MT1-MMP protein expression and hepatocellular carcinoma invasion and metastasis were analyzed. [Results] The expression of MT1-MMP protein in SHCC differs from that in advanced HCC remarkably （P = 0.007）, while, the MT1-MMP mRNA expression fails to show significandy difference between SHCC and advanced HCC （P = 0.693）. The expression of MT1-MMP protein has a positive correlation with tumor invasion and metastasis （P = 0.021, P = 0.001）. [ Conclusion ] The post-transcriptional regulation probably causes abnormal expression of MT1-MMP protein in hepatocarcinoma, which results in the differences of tumor biological behaviors between advanced HCC and SHCC.