摘要
目的探讨内毒素(LPS)诱导的急性肺损伤(ALI)大鼠肺组织甲状腺转录因子-1(TTF-1)、肺表面活性物质相关蛋白-A(SP-A)表达的变化。方法采用免疫组化和RT-PCR检测各组肺组织TTF-1、SP-A表达。结果ALI后12、24 h,肺组织中TTF-1、SP-A表达水平明显降低,且TTF-1蛋白变化与SP-A mRNA变化呈显著正相关。结论ALI大鼠肺组织TTF-1表达明显降低可能与ALI发生、发展有关。
Objective To investigate the change of the expression of TTF- 1 and SP - A in lung tissue of ALI rats induced by LPS. Methods The protein and mRNA of TTF- 1 and SP- A in lung tissue of ALI rats at different hours were measured with immunohistochemistry and RT- PCR method respectively, Results The expression of TTF- 1 and SP- A significantly decreased at 12 and 24 hours. A significant positive correlation between TTF- 1 and SP- A mRNA expression levels was detected, Conclusion The expression of TTF- 1 in lung tissue of ALI rats induced by LPS decreased, which may be related to the occurrence and development of ALI,
出处
《中国急救医学》
CAS
CSCD
北大核心
2005年第11期810-812,共3页
Chinese Journal of Critical Care Medicine