尼可地尔减轻大鼠供体肺缺血/再灌注损伤的实验研究

An experimental study on the effects of Nicorandil on ischemia-reperfusion injury of preserved rat lung

目的研究钾离子通道开放剂尼可地尔对低温保存后大鼠供体肺缺血/再灌注损伤的作用。方法24只健康雄性SD大鼠随机分为3组,A组为常规保存组,B组为尼可地尔(NCR)组,C组为尼可地尔+格列本脲(NCR+GLY)组。再灌注过程中每15 min测定动脉血氧分压,以评定移植体功能。实验结束时取肺组织测定丙二醛(MDA)的含量,测湿干比(W/D值)及观察超微结构改变。结果B组肺PvO2,W/D及超微结构改变显著优于A组和C组(P<0.05),同时,B组再灌注前、后肺组织MDA含量较A组和C组显著降低(P<0.05)。A组和C组各项指标无显著性差异,GLY阻断了NCR的有益作用。结论钾离子通道开放剂可减轻低温保存后的大鼠肺缺血/再灌注损伤。

Objective To study the effects of potassium channel openers Nicorandil on isehemia - reperfusion injury of preserved rat lung.Methods Twenty - four healthy male SD rats were randomly divided into 3 goups： control goup （goup A）, nicorandil goup （goup B）, nicorandil ＋ glibenclamide goup （goup C）. PaO2 was measured at intervals of 15 min during the reperfusion for evaluating the function of graft. The contents of MDA of graft tissue and wet - to - dry ratio were measured at the conclusion of the reperfusion period, and the changes of uhrastructure were observed. Results Lung PvO2, wet - to - dry weight ratio and uhrastructure changed more significantly in goup B, MDA concentrations of pulmonary tissue were significantly reduced in goup B before and after reperfusion compared with goup A and C（ P 〈 0.05 ）. Wet - to - dry weight ratio, MDA concentration of pulmonary tissue were not significantly different between the goup A and C, which meant glibenclamide eliminated the beneficial effects of nicorandil. Conclusion The ATP - sensitive potassium channel openers can attenuate isehemia - reperfusion injury of rat lung after hypothermic ischemia and storage.