中药成分对培养的鸡脾脏淋巴细胞增殖的影响

Effects of Chinese Herbal Medicinal Ingredients on Proliferation of Splenic Lymphocyte Cultured in Chicken

为筛选增强免疫作用较好的中药成分和研制新型复方中药成分免疫增强剂,比较了4种浓度的当归多糖(CAPS)、黄芪多糖(APS)、板蓝根多糖(IRPS)、淫羊藿多糖(EPS)、蜂胶多糖(PPS)、淫羊藿黄酮(EF)、蜂胶黄酮(PF)、黄芪皂苷(AS)和人参皂苷(GS)9种中药成分对培养的鸡脾脏淋巴细胞增殖的影响。结果表明,多数中药成分能促进淋巴细胞增殖,其中APS、EPS、GS和AS等在一定浓度既能单独又能协同ConA或LPS刺激淋巴细胞增殖,以EPS和APS作用最强。同时根据浓度试验结果,选出各药的合适浓度,进一步比较它们作用的差异,发现EPS和GS效果最好。这些中药成分可以作为复方中药成分免疫增强剂的组分药。

In order to select Chinese herbal medicine ingredient （CHMI） and develop a new-type compound CHMIs immunopotentiator, the effects of nine Chinese herbal medicinal ingredients, Chinese angelica polysaccharide （CAPS）, astragalus polysaccharide （APS）, isatis root polysaccharide （IRPS）, epimedium polysaccharide （EPS）, propolis polysaccharide （PPS）, epimedium flavone （EF）, propolis flavone （PF）, astragalosides （AS） and ginsenoside （GS）, at four concentrations on promoting splenic lymphocyte proliferation were compared in chicken. The results showed that most of CHMIs had better action, in which APS, EPS, AS and GS etc. could stimulate the lymphocyte proliferation singly or synergistically with ConA or LPS, the actions of EPS and APS were the strongest. At the same time, according to the above experimental results, one suitable concentration of each CHMI was chosen and their efficacies were further compared. It was found that the effects of EPS and GS were the best. These CHMIs would be expected as the component drugs of the new-type compound CHMIs immunopotentiator.