摘要
In order to amplify the partial bcl-2 gene of goose,we designed a pair of primers according to the reported chicken bcl-2 gene sequence in the GenBank,and obtained the gene by reverse transcript-polymerase chain reaction(RT-PCR).Then the gene was cloned into pMD-18T vector and identified by PCR.The nucleotide and amino acid sequences of goose partial bcl-2 gene were compared with the counterpart sequences of chicken,and the nucleotide homology was 92.49%.This is the first report of bcl-2 partial nucleotide sequence of goose.
In order to amplify the partial bcl-2 gene of goose, we designed a pair of primers according to the reported chicken bcl-2 gene sequence in the GenBank, and obtained the gene by reverse transcript-polymerase chain reaction （RT-PCR）. Then the gene was cloned into pMD-18T vector and identified by PCR. The nucleotide and amino acid sequences of goose partial bcl-2 gene were compared with the counterpart sequences of chicken, and the nucleotide homology was 92.49%. This is the first report of bcl-2 partial nucleotide sequence of goose.
出处
《畜牧兽医学报》
CAS
CSCD
北大核心
2005年第11期1228-1231,共4页
ACTA VETERINARIA ET ZOOTECHNICA SINICA
基金
国家自然科学基金项目(30300253)
中国博士后科学基金项目(2003033469)
