维生素E琥珀酸酯诱导MCF-7乳腺癌细胞的凋亡

Apoptosis of MCF-7 breast cancer cells induced by vitamin E succinate

目的检测维生素E琥珀酸酯(VES)对MCF-7乳腺癌细胞的增殖抑制和凋亡诱导作用,并分析凋亡诱导分子Fas表达的变化。方法人乳腺癌细胞株MCF-7[雌激素受体阳性,ER(+)]以VES刺激1 2,2 4 h和4 8 h,VES浓度为5μg/mL,1 0μg/mL和2 0μg/mL,用MTT法测定VES对细胞增殖的抑制作用;以流式细胞仪分析细胞周期和细胞表面Fas的表达;W estern蛋白印迹法检测VES作用后Fas蛋白水平的变化。结果VES对MCF-7乳腺癌细胞具有显著的抑制作用,并表现为时间和剂量依赖关系。MCF-7乳腺癌细胞的自然凋亡率为1.2%;5μg/mL,1 0μg/mL和2 0μg/mL的VES作用4 8 h后凋亡率分别升高至1 1.2%,1 6.4%和4 1.2%。VES作用后乳腺癌细胞Fas蛋白水平和细胞表面Fas表达升高。结论VES对ER(+)乳腺癌细胞具有显著的增殖抑制作用,并诱导细胞凋亡,其机制与细胞表面Fas表达上调有关。

Objective To investigate the growth inhibition and apoptosis induction effect of vitamin E succinate on MCF-7 human breast cancer cells and to analyze the modulation of Fas expression in this process. Methods Estrogen receptor positive MCF-7 human breast cancer cells were treated with VES for 12 h, 24h and 48 h. The concentrations of VES were 5 μg/mL, 10 μg/mL and 20 μg/mL. The inhibitory effect was measured with MTT method and the cell cycle and cell surface Fas expression were analyzed with flow cytometry. Fas protein level was detected by Western blotting assay. Results VES had significant inhibitory effect on the growth of MCF-7 human breast cancer cells and the effect was dependently related to time and dosage. The apoptotic rate rose from 1.2% to 11.2% ,16.4% , 41.2% ,after treated with VES for 48h at the concentrations of 5 μg/mL, 10μg/mL, 20μg/mL respectively. Fas protein level and cell surface Fas expression in cancer cells increased after the administration of VES. Conclusions VES had significant growth inhibition and apoptosis induction effect on MCF-7 estrogen receptor positive breast cancer cells. The mechanism was related to Fas upregulation on the surface of cancer cells.