FHITmRNA和WWOXmRNA在乳腺癌中的表达及其临床意义

Expression of FHITmRNA and WWOXmRNA in human breast cancer and their clinical significance

目的观察乳腺癌组织中FHITmRNA和WWOX mRNA表达情况,并探讨其与临床病理学指标的关系。方法以逆转录-聚合酶链反应(RT-PCR)法检测5 1例乳腺癌及癌旁组织标本中染色体脆性位点抑癌基因FHITmRNA和WWOX mRNA表达,半定量分析电泳结果。以免疫组化法检测乳腺癌石蜡标本中的雌激素受体(ER)、孕激素受体(PR)和HER-2基因状态。结果5 1例配对标本中,WWOX mRNA表达在乳腺癌组织及癌旁非癌乳腺组织中差异有显著性(P<0.0 1);FHIT基因的mRNA表达在乳腺癌组织及癌旁非癌乳腺组织中差异有显著性(P<0.0 1)。FHIT mRNA和WWOXmRNA在乳腺癌组织中的低表达呈正相关(rs=0.3 5 4,P<0.0 5)。FHIT mRNA和WWOX mRNA的表达与临床病理分期有关(P<0.0 5),WWOX mRNA的表达与腋淋巴结转移有关(P<0.0 5)。结论FHIT和WWOX基因系重要的候选抑瘤基因,与其他预后指标的联合检测可望对患者的治疗预后作出更准确的预测。

Objective To investigate the expression of FHIT mRNA and WWOX mRNA in human breast cancer tissues and its relation to clinicopathological and other molecular parameters. Methods With reference to the expression of β-actin, the expression of FHIT mRNA and WWOX mRNA was determined by reverse transcription-polymerase chain reaction （RT-PCR） in 51 breast cancer and adjacent breast tissue, and semi-quantitative analysis of band densities was performed. The protein expression of estrogen receptor （ ER ） , progesterone receptor （ PR ） , Her-2 gene in the 51 breast cancer lesions was detected by immunohistochemical method. Results FHIT mRNA and WWOX mRNA expression was significantly different in 54 breast cancer tissue compared to adjacent breast tissue （ P 〈 0.01 ） . The expression of FHIT mRNA and WWOX mRNA in 51 breast cancer tissue was reduced and was related to the clinicopathological stage （ P 〉 0.05 ） ; of FHIT mRNA and WWOX mRNA was related to axillary lymph node metastasis （ P 〈 0.05 ） . There are correlations between the low expression of FHIT mRNA and WWOX mRNA in breast cancer tissue. Conclusions FHIT and WWOX are candidate tumor suppressor genes. Detected coordinately with other molecular parameters, they can make the prognosis of therapy more accurate to predict.