大鼠门静脉高压症及梗阻性黄疸时细菌移位与黄嘌呤脱氢酶和黄嘌呤氧化酶的关系

Relationship among bacterial translocation, xanthine dehydrogenase and xanthine oxidase in portal hypertension and obstructive jaundice rats

目的探讨大鼠门静脉高压症(portal hypertension,PH)及梗阻性黄疸(obstructive jaundice,OJ)时,细菌移位(bacterial translocation,BT)与黄嘌呤氧化酶(xanthine oxidase,XO)、黄嘌呤脱氢酶(xanthine dehydrogenase,XD)之间的关系。方法将雄性SD大鼠60只随机分为对照组(A组),胆总管结扎组(B组)和门静脉缩窄组(C组),每组20只。术后第3周取肠系膜淋巴结、脾、肝组织及门静脉、腔静脉血细菌培养,测定门静脉压力(free portal pressure,FPP),及肠XO,XD活性水平。结果B组及C组细菌移位率明显高于对照组(P<0.01),对照组为12%,B组和C组分别为28%和54%;B组和C组空肠XO水平活性明显高于对照组(P<0.01),B组和C组门静脉压力也较对照组升高。细菌移位率与XO活性成正相关(r=0.603)。XD活性水平无显著差异。结论门静脉高压症及梗阻性黄疸时可发生细菌移位,可能与肠黏膜屏障被破坏通透性增强有关,肠壁XO水平活性增强引起肠黏膜屏障通透性增高有助于细菌移位发生。

Objective To study the relationship among bacterial translocation, xanthine dehyolrogenase and xanthine oxidase in portal hypertension and obstracfive jaundice rats. Methods Sixty rats were divided randomly into 3 groups： sham laparotomy （group A）, common bile duct ligation （group B） and portal vein stenosis （group C） after 3 weeks. Mesenteric lymph nodes, spleen, fiver, portal vein, vena cava and blood samples were collected for bacterial cultures. Portal pressure, intestinal XD and XO activity were measured. The mucosai ultrastructure in ileum was observed. Results The bacterial translocation rats in group B and group C were significantly higher than that in control （ goup A ） （ P 〈 0.01）. In goup A, it was 12%. In group B and group C, the incidence of BT increased to 28% and 54%. XO levels and portal pros.sure elevated in group B and group C. Them was correlation between the ineidence of bacterial translocafion and changes in the intestinal XO levels （ r = 0. 603 ）. Conclusion Bacterial translocation in portal hypertension and obstructive jaundice rats is in response to enhanced penmeability of intestinal mucosa, which is responsible for the increased XO level.