应用插入序列PCR进行大肠杆菌O_(157):H_7基因分型的研究

Study on the application of insert sequence PCR in genotyping of E.coli O_(157):H_7

目的对不同地区、不同宿主大肠杆菌O157H7分离株进行基因分型。方法应用插入序列PCR方法,对反应体系中dNTPs、引物以及镁离子浓度等反应条件进行优化。扩增产物通过琼脂糖凝胶电泳检测和分析。结果14株不同来源O157H7分离株根据插入序列PCR图谱可分为A、B、C、D四个基因型。不同地区O157H7菌株存在不同的基因型;同一地区、毒力基因谱相同的O157H7菌株基因型也不相同;不同宿主O157H7菌株也存在相同的基因型。结论插入序列PCR技术用于大肠杆菌O157H7的基因分型,简便、快速、敏感,对大肠杆菌O157H7的分子流行病学研究具有重要价值。

Objecliwe To perform genetic categorization of Ecoli O157：H7 strains acquired from hosts in different districts. Methods IS - PCR was performed and PCR conditions were optimized, Amplification products were analyzed by agarose gel electrophoresis. Results A total of 14 strains of O157 ： H7 were further categorized as genetic groups A, B, C and D. Both strains from different regions and strains with similar virulence gene profiles in the same region could be fiu＇ther differentiated by IS - PCR, while strains isolated from different individuals may share the same profile. Conclusions IS - PCR is a fast sensitive method for Ecoli O157 ： H7 genetic categorization. It may possess significance in the research work of Ecoli O157：H7 molecular epidemiology.