油梨种质的离体低温保存

Preservation of Avocado in vitro Under Low Temperature

以哈斯油梨（ＰｅｒｓｅａａｍｅｒｉｃａｎａＭｉｌｌｃｖ．Ｈａｓｓ）为试材，从试管苗下胚轴诱导产生愈伤组织，研究了愈伤组织的离体低温保存技术。结果表明，采用８℃低温，愈伤组织能在ＭＳ＋１．０ｍｇ·１^(-１)２，４一Ｄ＋３％椰乳＋３％蔗糖固体培养基中保存６个月；在培养基中添加０．４ｍｇ·１^(-１)多效唑能减少继代次数，使保存期延长至８个月。

Calli derived from the hypocotyl of avocado (Persea americana Mill cv.‘Hass')were stored at 8℃invitro. The results indicated that the calli when cultured on MS medium + 1. 0mg·1￣(-1)2,4一D+ 3%coconutmilk + 3%sucrose and stored for 6 months still showed comparatively higher viability.The duration ofpreservation might be extended to 8 months by adding 0.40 mg·1￣(-1)paclobutrazol.