丹参对槟榔碱诱导血管内皮细胞凋亡的保护作用

The effects of Salvia Miltiorrhizae on the apoptosis of vascular endothelial cells induced by arecoline

目的:探讨丹参对槟榔碱诱导血管内皮细胞凋亡的保护作用。方法:体外培养人脐静脉内皮细胞(Vein Endothelial Cells,VEC)为模型,槟榔碱作为致伤因素,丹参作为保护因素,用Hoechst 33258染色,在荧光显微镜下观察凋亡细胞核形态学改变并计算凋亡百分率。结果:正常培养的血管内皮细胞凋亡较少,槟榔碱刺激后可见到明显的细胞凋亡,丹参预处理后则凋亡细胞明显减少,凋亡率与槟榔碱组相比有显著性差异(P<0.05)。结论:丹参对槟榔碱诱导的VEC凋亡有一定的保护作用。

Objective： To explore the protective effects of Salvia Miltiorrhizae （SM） on the apoptosis of vascular endothelial cells（VEC） induced by arecoline. Method： Human Umbilical Vein Endothelial Cells（HUVECs） were cultured in vitro. Apoptosis of VEC were induced by arecoline, and SM was added in test specimen. Apoptotic morphological changes and percentage of apoptosis were assayed with Hoechst 33258 staining and were observed under fluorescent microscope. Result：There was relatively less number of apoptosis of in vitro cultured normal VEC than that after arecoline stimtdation. After treatment with SM, VEC apoptosis decreased significantly（P〈0.05）. Conclusion. SM might exert protective effects on VEC against apoptosis induced by arecoline.