青蒿素对血管平滑肌细胞增殖的影响

Effect of artemisinin on the proliferation of cultured vascular smooth muscle cell

目的 :观察青蒿素 (artemisinin ,Art)对体外培养大鼠主动脉平滑肌细胞 (VSMC)的增殖、DNA合成及细胞周期的影响 ,探讨作用机制。方法 :体外培养大鼠主动脉VSMC ,分为不同浓度的Art组及对照组。观察细胞生长曲线 ;四甲基偶氮唑盐微量酶反应 (MTT)法检测细胞活性 ;3H 胸腺嘧啶脱氧核苷(3H- TdR)掺入法观察其对DNA合成的影响 ;通过HE染色、DNA梯带检测和流式细胞术观察细胞的凋亡并测定细胞周期。结果 :与对照组相比 ,各浓度Art组VSMC计数不同程度减少 ,3H- TdR掺入率降低 ,细胞增殖受抑制 ,VSMC凋亡增加 ,并呈浓度依赖性 ;Art诱导细胞凋亡 ;使细胞周期阻滞于G0 / G1 期。结论 :Art对体外培养VSMC的增殖有抑制作用 ,可能通过抑制细胞DNA合成 。

Objective：To observe the effects of Artemisinin （Art） on the proliferation of cultured rats vascular smooth muscle cell （VSMC） in vitro and analyses the mechanism. Method： VSMC was isolated from rat＇s aorta and cultured with different concentration of Art. Growth curve of VSMC was recorded, DNA replication were evaluated by [^3 H]-TdR method. Flow metometry was used to analyze cell apoptosis and cell cycles. Result：Comparing to the control group, VSMC proliferation of Art interfering groups were inhibited, and [^3H]-TdR intermingle rate were decreased as well as cell apoptosis were induced. The cell cycle were blocked at G0/G1 phase by Art in a dose dependent. Conclusion： Art inhibits VSMC proliferation by disturbing DNA replication, cell apoptosis and blocking cell cycle at G0/G1 phase.