胃癌中p16基因缺失与突变的研究

Deletion and mutation of p16 gene in primary gastric carcinoma

目的探讨p16基因外显子2的缺失和突变与胃癌发生发展的关系。方法应用新鲜组织标本基因组DNA抽提、PCR-SSCP分析的方法,对30例胃癌及癌旁组织中p16基因外显子2的缺失和突变进行检测。结果胃癌组织样本中p16基因外显子2的缺失率为10.00%,突变率为10.00%,两者之和为20.00%,癌旁组织样本中未发现缺失和突变,统计学分析有显著性差异(P<0.01)。结论p16基因外显子2的缺失和突变与胃癌的发生具有一定的相关性。

Objective ：To study the relationship between the deletion and mutation of p16 gene in exon 2 and the occurrence of primary gastric carcinoma. Methods： Using molecular biological methods that include genome DNA extraction from fresh tissue and PCR-SSCP analysis technique, it was studied that the deletion and mutation of p16 gene in exon 2 in 30 cases of primary gastric carcinomas. Results：The total alteration rate of p16 gene in exon 2 in primary gastric carcinomas was 20. 00% （6/30） in which the deletion rate was 10. 00% （3/30） and the mutation rate was 10. 00% （3/30）. In contrast, neither deletions nor mutations was found in the control group of adjacent gastric mucosa, respectively with significant difference（ P 〈 0. 01 ）. Conclusion ：The deletion and mutation of p16 gene in exon 2 is related to the genesis of primary gastric carcinoma.