新辅助化疗对乳腺癌细胞凋亡和增殖的影响

Effects of neoadjuvant chemotherapy on apoptosis and proliferation of breast cancer

目的观察乳腺癌新辅助化疗后癌细胞凋亡及增殖程度的变化,判断化疗疗效。方法新辅助化疗手术标本44例和术前未接受化疗的对照组37例,进行Ki67及PCNA免疫组织化学染色和TUNEL原位细胞凋亡检测,并进行统计学处理。结果化疗组Ki67、PCNA和AI的阳性细胞数比率分别为11.8%、31.3%和69.8%,对照组分别为21.6%、62.8%和52.8%,化疗组和对照组相比均有显著性差异。结论新辅助化疗能抑制乳腺癌细胞的增殖,诱导凋亡。

Objective： To observe the change of the apoptosis level and proliferate degree of breast carcinomas after preoperative chemotherapy. Methods ： Immunohistochemical labeling performed with the Ki67 and PCNA monoclonal antibodies, and TUNEL （terminal-deoxynucleotidyl transferase mediated d-UTP nick end labeling） were used. Results：The expressions of Ki67, PCNA in preoperative groups （ 11.8%, 31.3% ） were significantly lower than those in control groups （21.6%, 62. 8% ）. Apoptosis index of preoperative group （69.8%） was higher than that in control group （52. 8% ）. Conclusion：The study showed neoadjuvant chemotherapy inhibited the proliferatation of breast cancer cell and induced apoptosis.