急性白血病类胰蛋白酶与血管内皮生长因子的相关性研究

Tryptase Relation to VEGF in Acute Leukemia

为了探讨类胰蛋白酶在急性白血病血管新生中的作用,应用免疫细胞化学方法检测了61例急性白血病患者白血病细胞的类胰蛋白酶及血管内皮生长因子(VEGF)的表达情况,并对二者之间的关系进行了分析。结果表明:51例急性髓细胞白血病中(M13例,M215例,M320例,M45例,M58例)有15例患者的白血病细胞类胰蛋白酶表达为阳性,阳性率为29.4%,其中包括M11例,M27例,M35例,M52例。10例急性淋巴细胞白血病患者类胰蛋白酶表达均为阴性。急性髓细胞白血病患者类胰蛋白酶阳性的细胞数与其年龄、外周血白细胞数、外周血和骨髓原始细胞数以及中性粒细胞过氧化物酶之间没有相关性。急性髓细胞白血病患者中有41例VEGF表达阳性(80.4%);急性淋巴白血病患者有3例VEGF表达阳性(30%)。急性髓细胞白血病M2亚型的患者中,类胰蛋白酶与VEGF的表达均明显增高且二者之间具有显著相关性(r=0.65,P<0.05)。结论:类胰蛋白酶可以作为急性髓系白血病的特异性标记物,它可能参与了急性髓细胞白血病M2亚型的血管新生。

In order to investigate the role of tryptase in angiogenesis of acute leukemia （ AL）, the expressions of tryptase and vascular endothelial growth factor （VEGF） in leukemic cells from 61 patients with AL were examined by using immunocytochemical method, and the correlation between tryptase and VEGF was analyzed. The results showed that tryptase positive expression was found in 15 out of 51 patients with acute myeloid leukemia （AML） （M1 1/3, M2 7/15, M3 5/20, M5 2/8 ）. Tryptase positive expression was 29.4% in AML. However, none of 10 patients with acute lymphocytic leukemia （ALL） showed tryptase expression. There were no correlations between the amounts of cells with tryptase expression and patient age, WBC count, numbers of blood or marrow myeloblasts and neutrophil POX. VEGF expression was revealed in 41 patients with AML （ 80.4% ） and only 3 with ALL （ 30% ）. Significant correlation has been found between the expression of tryptase and that of VEGF in AML-M2 （ r = 0.65, P 〈0.05 ）. It is concluded that tryptase appears to be a myeloid-specific marker in AML and may be involved in the angiogenesis of AML-M2.