人类ERMAP基因在不同细胞系中表达的研究

Expression of Human ERMAP Gene in Different Cell Lines

为了探讨人类ERMAP基因在不同细胞系的表达谱和表达量的特点,选择血液肿瘤、实体瘤、正常组织细胞等不同类型的15种细胞系,在培养的12、24、36、48、60、72小时共6个时间点采集细胞,用荧光定量PCR检测人类ERMAP基因表达量。结果发现,K562细胞在培养12、24小时时ERMAP基因表达阳性,其表达量分别为(5.092±2.331)×106cps/μlRNA、(5.328±3.916)×106cps/μlRNA;ECV304细胞在培养24小时时ERMAP基因表达阳性,其表达量为(0.84±0.12)×106cps/μlRNA,其余13株细胞系ERMAP基因表达阴性。结论:首次发现人类ERMAP基因在ECV304细胞系中表达,进一步证实ERMAP在K562细胞系中表达。

To investigate the pattern of human ERMAP gene expression in different cell lines, 15 cell lines derived from hematopoietic tumor, somatic tumor and normal tissue were chosen and were cultured; cells were harvested after culture for 12,24,36,48,60 and 72 hours; the expression of the human ERMAP was detected by using fluorescent quantitative PCR, The results showed that human ERMAP gene expression was positive in K562 cell line at interval of 12 and 24 hours, and the expression levels were （5. 092 ± 2. 331 ） ×10^6 cps/ixl RNA, （5. 328± 3. 916） ×10^6 cps/μl RNA, respectively; ERMAP gene expression was also positive in ECV304 cell line at interval of 24 hours, and the expression level was （0.84±0. 12） ×10^6 cps/μl RNA; and its expression was negative in other 13 cell lines. It is concluded that human ERMAP gene expression in ECV304 cell line was found first, and its expression in K562 cell line was further confirmed,