rhG-CSF体内应用对骨髓和外周血造血干/祖细胞上CXCR-4表达的影响

In Vivo Effect of rhG-CSF on the CXCR-4 Expression of Hematopoietic Progenitor or Stem Cells in Bone Marrow and Peripheral Blood

为了观察rhGCSF动员对外周血和骨髓造血干/祖细胞上CXCR4表达的影响,应用三色荧光标记技术对动员前后骨髓和外周血中单个核细胞(MNC)和CD34+细胞上CXCR4的表达进行了测定。结果显示,GCSF动员显著增加了外周血MNC及骨髓CD34+细胞上CXCR4的表达,骨髓MNC及外周血CD34+细胞上CXCR4的表达无变化,稳态骨髓(SSBM)中CD34+细胞比例与GBM、GPB中CD34+细胞比例呈正相关,与采集到的骨髓及外周血中CD34+细胞/公斤也具有良好的正相关性。结论:GCSF体内应用后CD34+细胞上CXCR4表达的变化可能是动员机制的一部分,CXCR4的高表达可能有利于MNC的植入,SSBM中CD34+细胞的含量可作为动员效果好坏的预测指标。

To investigate the effect of in vivo rhG-CSF on the CXCR4 expression of hematopoitic progenitor or stem cells in bone marrow and peripheral blood, the expressions of CXCR-4 on CD34^＋ cells and mononuclear cells of bone marrow and peripheral blood from healthy donor before and after mobilization were detacted by three-color fluorescence analysis. The results showed that a significantly higher expression of CXCR4 on CD34^＋ cells of bone marrow and mononuclear ceils of periperal blood, as compared to those before mobilization. There were no significant differences of CXCR4 expresssion of CD34^＋ cells in peripheral blood after mobilization, as compared with steady state bone marrow, and no dynamic change of mononuclear cells expressing CXCR-4 in bone marrow before and after mobilization. Significant positive correlation were found between the percentage of CD34^＋ cells in bone marrow before mobilization and that in bone marrow and peripheral blood after mobilization ; furthermore, the percentage of CD34^＋ cells of bone marrow before mobilization had a positive correlation with both the count of CD34^＋ cells per kilogram on the day of collection in bone marrow and peripheral blood after mobilization. It is concluded that the mobilization of hematopoitic cells may be involved in the signaling of SDF-1/CXCR4 according to the increase of the surface expression of CXCR4 on CD34^＋ cells in bone marrow and on the MNC in peripheral blood after mobilization; meanwhile, the high surface expression of CXCR4 may contribute to the MNC engraftment, monitoring the percentage of CD34^＋ cells in bone marrow before mobilization can be regarded as a predictive factor for mobilization outcome.