实验动物皮肤病原真菌核酸鉴定方法的建立

The Development of the Assays for the Identification of Pathogenic Dermatophytes Nucleic Acid in Laboratory Animals

目的选择一种快速、敏感和特异的方法来检测实验动物皮肤病原真菌。方法选取真菌CHS1基因中一段序列设计皮肤真菌通用引物和选取一种随机引物FM1,利用聚合酶链反应(PCR)扩增三种动物皮肤病原真菌标准株:须毛癣菌、石膏样小孢子菌、犬小孢子菌。结果两种引物扩增产物片段大小在三种真菌之间有明显不同。结论本实验方法可快速特异性检测实验动物皮肤病原真菌。

Objective To develop a method with high sensitivity, specificity and rapidity to detect pathogenic dermatophytes in laboratory animals. Methods One fragment of CHS1 gene was selected as universal primer of dermatophytes in this PCR amplification. Otherwise, a random primer FM1 was also used to amplify three dermatophytes species standard strain： Trichophyton mentagrophyton, Microsporum gypseum, Microsporum canis. Results There were distinguishable fingerprinting patterns among three dermatophytes species. Conclusion The test could detect some common dermatophytes species of laboratory animal rapidly and specifically.