损毁大鼠下丘脑室旁核后AVP和ANP的关系

Relationship of argininvasopressin and atrial natriuretic peptide after damage of paraventricular nucleus of hypothalamus

目的借助立体定向技术尝试建立因下丘脑室旁核损伤引起的中枢性尿崩症动物模型。在此基础上探讨下丘脑室旁核调节水钠代谢的机制。方法SD大鼠在立体定向仪引导下,试验组向下丘脑室旁核(PVN)注射海人藻酸,以达到损毁目的;对照组则注射人工脑脊液,取血检测血管加压素(AVP)和心钠素(ANP)水平。对比分析尿量,研究PVN损伤后AVP和ANP在体内的变化。结果下丘脑PVN损毁后试验组大鼠尿量为(1.26±0.34)ml,明显多于对照组(0.78±0.16)ml;检测血浆AVP和ANP含量分别为试验组(3.91±0.48)pg/ml、(332±27.48)pg/ml对照组(8.05±0.78)pg/ml、(291±31.66)pg/ml。结论下丘脑PVN损毁后血浆AVP水平降低,而ANP水平反而升高,该结果可能是下丘脑损伤后机体水钠代谢紊乱的深层原因,即具体表现为肾脏排水排钠增加。

Objective To damage paraventricular nucleus （ PVN ） of hypothalamus and develope a central diabetes insipidus animal model with stereotactic technique, then to carry out the research of water and sodium metabolic regulation based on this model. Methods Toxic amino acid was injected into PVN in experiment＇s group and artificial cerebrospinal fluid was injected into PVN in control group. Urine volume in one and a half hour after microinjectiou was collected, and 2 ml blood samples were drawn from eyeball. Plasma argininvasopressin （AVP） and atrial natriuretic peptide （ANP） were detected with radioiuununoassay（RIA） to analyze urine volume and the level of AVP and ANP. Results The results from above-mentioned illustrated that urine volume （1. 26±0. 34） ml increased in experimental group comparing with that in control group （0.78 ± 0. 16） ml. The level of plasma AVP was （3.91±0.48） pg/ml in experiment group and （8.05 ±0.78）pg/ml in control group, while the level of plasma ANP was （332 ±27.48） pg/ml in experiment and （291 ±31.66） pg/ml in control. Conclusion After PVN is damaged, the level of AVP in plasma decreases and the level of ANP in plasma increases. These pathophysiological results may be a main reason for metabolic regulation disturbance after the PVN of hypothalamus is damaged.