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广西巴马小型猪IL-4和IL-10基因的克隆和序列分析 被引量：6

Cloning and Sequence Analysis of Interleukin 4 and Interleukin 10 of Bama mini pig
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摘要 为研究猪白细胞介素4(IL-4)和白细胞介素10(IL-10)在机体免疫应答中的作用,从ConA刺激的巴马小型猪的外周血淋巴细胞提取总RNA,用RT-PCR方法扩增出IL-4和IL-10基因,将其分别克隆到PMD18-T 载体上,进行序列分析.结果表明,克隆的IL-4和IL-10基因的核苷酸和氨基酸序列与GenBank上登录的其它猪种的IL-4和IL-10基因的核苷酸和氨基酸序列同源性分别为98.8%～100%、97.0%～99.3%和99.2%、97.7%. In order to understand the functions of interleukin 4 （IL-4） and interleukin 10 （IL-10） in immune system, the lymphocytes of Bama mini pig was separated from peripheral blood and the total RNA was extracted from the peripheral blood lymphocytes stimulated with ConA, respectively. The genes of IL-4 and IL-10 were amplified by RT-PCR and cloned into PMD18-T vector, respectively. The sequences analysis showed that the nucleotide and the deduced amino acid sequences of the IL-4 and IL-10 in this study share 98.8%-100%, 97.0%-99.3% and 99.2%, 97.7% homology with those of other pigs,respectively.
作者 李军 熊艳芸 梁湘 廖素环 罗廷荣
机构地区 广西大学动物科学技术学院
出处 《动物医学进展》 CSCD 2005年第10期73-77,共5页 Progress In Veterinary Medicine
基金 广西大学科学技术研究重点基金资助项目(2003ZD01) 广西科技攻关项目(桂科攻0480001) 广西科学研究与技术开发计划项目(桂科攻0480001)
关键词 白细胞介素4 白细胞介素10 基因克隆 序列分析 广西巴马小型猪 interleukin 4 interleukin 10 gene cloning sequence analysis Bama mini pig
分类号 Q503 [生物学—生物化学] S858.28 [农业科学—临床兽医学]
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参考文献20

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二级参考文献23

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动物医学进展
2005年 第10期

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