摘要
为了观察在发生内毒素血症时,山羊红细胞膜上Na+-K+-ATP酶活性以及红细胞内和血清中K+离子浓度的变化,将体重10 kg±1 kg的12只山羊,随机分为内毒素处理组(LPS,1 mg/kg)和生理盐水对照组。每组分别在处理后第0,0.5,1,2,3,4,5小时和第6小时从颈静脉采取血液样品。采血之后,制备红细胞、红细胞膜和血清。检测红细胞膜上Na+-K+-ATP酶活力,红细胞内和血清中K+浓度。结果表明,发生内毒素血症时,红细胞膜上Na+-K+-ATP酶活力和红细胞内K+离子浓度都先升高后降低,血清中K+离子浓度先降低后升高。
The purpose of this experiment was to determine the effect of endotoxin（LPS） on Na^＋-K^＋-ATPase and explore the action mechanisns of LPS in the goats with endoxemia. The healthy goats（10 kg±1 kg） were randomly divided into two group： saline group（n=6） and LPS group（n=6）. The saline group and LPS group received saline and Lipopolysaccharide （1 mg/kg ） through neck vein, respectively. The blood samples were collected at 0, 0.5, 1, 2, 3, 4, 5 h and 6 h after treatment,then separated red blood cell and serum from the sample and made red blood cell membrane. Na^＋-K^＋-ATPase in the red blood cell membrane and the K^＋ concentration in the red blood cell and serum were measured. It showed thatNa^＋-K^＋-ATPase （LPS group） initially became more active, then repressed. Concentration of K^＋ in the red blood cell initially increased, then decreased. On contrast, concentration of K^＋ in the serum initially decreased and then increased.
出处
《动物医学进展》
CSCD
2005年第10期97-100,共4页
Progress In Veterinary Medicine
