大肠杆菌高效表达的HCV E_2蛋白在丙型肝炎检测中应用

Detection of the antibody of second envelope glycoprotein expressed by E. coli in HCV infection

为探讨大肠杆菌高效表达的HCV E2蛋白在丙型肝炎诊断中应用价值,采用了238份HCVRNA阳性血 清标本进行了E2抗体和抗HCV测定,方法采用ELA,和PCR法。结果有164份E2抗体阳性,占68．9％,卡方检验P >0．05;用抗HCV检测与E2抗体测定方法比较,两种方法无显著性区别(P>0．05);在HCV RNA阳性,抗HCV阴性 的血清中,有半数可测出E2抗体的阳性。提示在大肠杆菌中高效表达了可溶性的HCV E2蛋白,纯化后可进行E2 抗体测定,且E2抗体阳性数与HCVRNA,抗HCV阳性数无显著性差别,E2抗体可以检出一部分抗HCV检测不能检 出的HCV感染者。

To explore value of the antibody of second envelope glycoprotein of hepatitis C virus expressed by E. coli in the diagnosis of HCV infection. 238 positive serum samples of HCVRNA were detected for anti - E2 and anti - HCV with ELISA. Anti - E2 positive rate was 68,9%, Anti - HCV positiverate was 68.2%. There was no significant difference（ P 〉 0.05） In 77 anti - HCV negative sera 50% was anti - E2 positive. These results show that the HCV E2 glycoprotein expressed by E. coli can be used as antigen for detecting the antibody E2, as a complement of anti - HCV detection in diagnosis of HCV infection