慢性乙肝患者肝组织纤维化程度与细胞因子相关性

The relationship between serum cytokine and liver fibrosis in the patients with chronic hepatitis B

探讨慢性乙肝患苦血清细胞因子(TGF-β1、TNF-α、IFN-γ)在肝纤维化中的作用机制。采用ELISA和 RIA方法检测95例慢性乙肝患者血清TGF-β1、TNF-α、IFN-γ、HA、PCⅢ、C-Ⅳ的水平,并与肝组织病理学变化进 行对照研究。结果显示慢性乙肝患者血清TGF-β1、TNF-α、IFN-γ、HA、PCⅢ、C-Ⅳ的含量均不同程度高于对照 组,且随肝损害程度的加重而升高,与肝损害程度呈正相关;并且其水平与肝纤维化程度具有明显的相关性(P< 0．05)。血清TGF-β1水平与血清HA、PCⅢ、C-Ⅳ水平具有直线相关性,而IFN-γ与血清HA、PCⅢ、C-Ⅳ水平呈 负相关。慢性乙肝患者血清TCF-β1、TNF-α、IFN-γ水平可以作为诊断肝纤维化的血清学指标。

To explore the relationship between serum cytokines and liver fibrosis in patients with chronic hepatitis B（CHB）, and discuss their role in the pathogenesis of liver fibrosis. ELISA and RIA techniques were used to detect the levels of transforming growth factor - β1 （ TGF - β1 ）, tumor necrosis factor - α （TNF - α）, interferon - γ （ IFN -γ ）, hyaline acid （HA）, type Ⅲ procollagen （PC Ⅲ） and collagen type Ⅳ （ C - Ⅳ ） in 95 patients with CHB, and contrast study were performed between those serum markers and hepatic pathology, levels serum TGF - β1, TNF - α, HA, PC Ⅲ and C - Ⅳ in the patients with CHB were significantly higher than those in normal control group（ NC）, and were positively correlated with degree of liver damage and hepaticfibrosis the serum IFN- γ level was negative Correlated with HA., PC Ⅲ and C - Ⅳ .The serum levels of TGF- β1 ,TNF- α and IFN - γ of the patients with CHB were closely correlated with liver fibrosis. Serum TGF - β1, TNF- α and IFN - γ levels are helpftd to diagnose liver fibrosis.