摘要
目的:探讨日达仙诱导肾癌细胞株ACHN凋亡的抗肿瘤作用的机制.方法:以浓度50～400 mg/L日达仙作用肾癌ACHN细胞24～72 h后,用MTT比色法检测细胞生长活性,用单细胞凝胶电泳法和流式细胞仪分别检测ACHN细胞DNA损伤和凋亡的情况.结果:日达仙能显著抑制肾癌细胞株ACHN细胞的体外生长,呈时间与剂量依赖性;日达仙诱导肾癌细胞株ACHN凋亡.结论:日达仙通过抑制肾癌细胞株ACHN增殖、诱导细胞凋亡等机制,显著抑制肾癌细胞的体外生长.
Objective： To study the in vitro apoptosis effects of Thymosin α1 on renal carcinom cell line ACHN. Methods：After being treated with 50-40 mg/L Thymosin α1 for 24-72 h, the growth activities of cancer cells were studied by MTT. Single cell gel electrophoresis（SCGE） and flow cytometery （FCM） were used to detect DNA damage and apotosis of renal carcinoma cell line ACHN. Results： The growth of renal carcinoma cell line ACHN was inhibited by Thymosin m in a time dependent and dose dependent manners in vitro. Apoptosis of renal carcinom cell line ACHN can be induced by Thymosin α1 in vitro. Conclusions： Thymosin αlcould significantly in hibit the growth of renal carcinoma cell line ACH by blocking cellular proliferation and inducing apoptosis.
出处
《临床泌尿外科杂志》
2005年第10期637-639,共3页
Journal of Clinical Urology
关键词
日达仙
肾肿瘤
细胞凋亡
Thymosin α1
Renal tumour
Cell apoptosis
