腺病毒介导CDES融合基因对乳腺癌细胞的抑制作用

The Inhibitory Effect of CDES Gene on Mammary Cancer Mediated by Adenovirus

目的:构建一种重组腺病毒rAdCDES,在体内外进行抑瘤和放疗增敏作用实验研究。方法:用细菌内同源重组法与腺病毒骨架质粒重组构建出重组腺病毒质粒,经脂质体介导转染293包装细胞扩增获取重组腺病毒rAdCDES。用测定生长曲线和MTT法检测其对人乳腺癌细胞株MCF-7的生长抑制情况;建立津白号鼠乳腺癌模型,观察rAdCDES和放疗对瘤体大小及鼠存活期的影响。结果:成功构建了rAdCDES;rAdCDES对MCF-7细胞生长抑制率达(83.1±8.1)%,与对照rAdLacZ (19.2±7.8)%相比较有显著性差异(P<0.01);体内rAdCDES对小鼠乳腺癌MA737细胞有明显生长抑制作用,延长了小鼠的存活期,且对放疗有肯定的增敏作用。结论:本文所构建的rAdCDES在体内外对乳腺癌细胞均有明显的抑制作用和对放疗的增敏作用。

Objective： To construct a recombinant adenovirus（rAdCDES） which is capable of both direct and indirect treatment to mammary cancer and enhancement to the antitumor effect of radiation. Methods： A method of homologous recombination in bacteria was used to construct prAdCDglyES. The recombination adenovirus was transfected to 293 cells by liposome, in which rAdCDES was packaged and generated. The growth curve and MTT methods was used to detect the growth inhibition effect of rAdCDES on MCF-7;rAdCDES was directly injected into established MA737 tumors-beating mice for observing difference in tumor size and survival days of mice and enhancement of the antitumor effect of radiation. Results： The inhibiting rate of rAdCDES on MCF-7 cell was （83.1 ± 8.1 ）% and had significant difference compared with control was （ 19.2 ± 7.8） % （P 〈0.01 ）. We observed also that there was a significant difference in tumor size and survival days in mice between the therapy group and control group and rAdCDES had enhancement of the antitumor effect of radiation. Conclusion： rAdCDES is capable of growth inhibition effect on mammary cancer in vivo and vitro and enhancement of the antitumor effect of radiation.