体外研究CpG基序对人食管癌疫苗的佐剂效应

Adjuvant Effects of CpG Motif-Containing Oligodeoxynucleotide in vitro on Human Esophageal Tumor Vaccine

目的:探讨CpG基序在人食管癌Eca-109细胞疫苗研究中的佐剂效应。方法:以Eca-109细胞膜表面小肽刺激DC作为食管癌疫苗,HL-60细胞膜表面小肽刺激DC作为对照疫苗。以CpG基序作为佐剂,以CBPw作为对照佐剂,测定各组的T细胞增殖试验和CTL活性。结果:佐剂组的T细胞活性明显高于其余各组(P<0.01);人食管癌疫苗组的T细胞活性明显高于其余各组(P<0.01);与Eca-109细胞匹配的HLA也影响了T细胞活性的发挥。结论:CpG基序是人食管癌疫苗的有效佐剂,HLA对T细胞功能有影响。

Objective： To investigate the adjuvant effects of CpG motif-containing oligodeoxynucleotide （CpG） in vitro on human esophageal cancer vaccine. Methods： Matured dendritic cells （ DC ） activated by peptides on Eta-109 cell membranes were used for human esophageal tumor vaccine and matured DC activated by peptides on HL-60 cell membranes were used for control vaccine. With adjuvant of CpG or the cell wall skeleton of coryne bacterium parvum （ CBPw）, we analyzed each group＇s T-cell proliferation and cytotoxic T lymphocyte （CTL） response. Results： The adjuvant of CpG or CBPw markedly enhanced T-cell proliferation and CTL response（ P 〈 0.01 ） ; The groups with human esophageal tumor vaccine had markedly greater T-cell proliferation and CTL response than other groups （ P 〈 0.05 ） ; The HLA gene locus alleles to match Eta-109 cell gene affected the activity of T cells. Conclusion： CpG may be the optimal adjuvant of human esophageal tumor vaccine and the HLA gene locus may affect T cells function.