对氧磷酶-1与代谢综合征的关系研究

Study on the relationship between paraoxonase-1(PON-1) and metabolic syndrome

目的:探讨对氧磷酶-1(PON-1)与代谢综合征(MS)的关系。方法:本文测定31例MS患者和33例健康人血清PON-1活性,并应用聚合酶链式反应—限制性片段长度多态性(PCR-RFLP)技术研究其PON-1第191位密码子基因变异。结果:(1)MS组血清PON-1活性低于对照组,差别具有统计学意义(t=-3.6@P<0.05)。(2)MS组与对照组PON-1第191位密码子基因型分布无显著性差异(χ2=0.922@P>0.05)。结论:MS组血清PON-1活性显著低于对照组,这可能是MS患者心血管疾病高发的原因之一。

Objective： To reveal the relationship between serum PON-1 activity, PON-1 Q/R polymorphism and metabolic syndrome in Chinese Han ethnic group. Methods： The subjects of the study consisted of two groups： （1） metabolic syndrome group; （2） normal-control group. Serum PON-1 activity was determined using paraoxon as substrate by measuring the increase rate in the absorbance at 405 nm due to formation of 4-nitrophenol, and PON-1 Q/R polymorphism was evaluated by polymerase chain reaction and restriction fragment length polymorphism （PCR-RFLP）. Results： （ 1 ） There was no significant difference in the distribution of the PON-1 Q/R polymorphism between metabolic syndrome group and normal- control group （X^2=0.922, P〉0.05）. （2） Serum PON-1 activity in metabolic syndrome group and normal- control group was （52.67±20.83）u/mland （74.85＋-27.72）u/ml, respectively.SerumPON-1activityintheformer group was significantly low compared with the control group （t= -3.6, P 〈0.05）. Conclusion： The present study suggested that serum PON-1 activity in metabolic syndrome group was significantly lower than fhe control group. Perhaps it was one of the reasons why MS patients were highly complicated with cardiovas- cular diseases.