抗Ki-67单克隆抗体识别的大肠癌细胞动力学分析

Cyto-kinetics analysis on colorectal carcinoma recognized by Anti-ki-67 monoclonal antibody

目的:探讨大肠癌细胞动力学特征及其临床意义。方法:30例大肠癌手术切除标本的癌、癌旁2cm、5cm及远切端大肠组织以抗ki-67单克隆抗体识别后应用流式细胞术测定细胞动力学指标。结果:(1)大肠癌细胞的异倍体率与癌旁2cm无显著差异,显著高于癌旁5cm及远切端大肠组织,(χ2=31.27P<0.01)。(2)大肠癌细胞的合成期百分数(SphasefractionDSPF)与癌旁2cm无显著差异,显著高于癌旁5cm及远切端大肠组织(F=34.99P<0.01)。(3)大肠癌组织增殖指数(ProliferationIndexDPI)显著高于癌旁2cm、5cm及远切端大肠组织,癌旁2cm显著高于远切端大肠组织(F=31.9696P<0.05)。(4)远端大肠癌细胞的异倍体率显著高于近端(P<0.001)。结论:(1)大肠癌组织的增殖水平高于癌旁及正常大肠组织。(2)癌旁2cm增殖活性亦高于正常大肠组织,癌旁2cm能否作为手术切除范围的低限尚值商榷。(3)近远端大肠癌增殖活性存在不同,远端大肠癌增殖活性更高,更具侵袭性。

Objective： To Analyze cyto-kenetics character of colorectal carcinoma and its clinic value. Methods： 30 samples of colorectal carcinoma（T）, tissue surrounding tumor 2cm （P2）and 5cm（P5）away from tumor margin and distal colonal tissue（N）were collected from surgical procedure, the preliferative activity（SPF,PI）and ploidy were assayed by ki-67-DNA two parameter FCM. Results： （1）The aneuploidy in T showed no difference with P2,both of them were significant higer than in P5 and N （X^2=31.27 P〈0.01 ）, （2） The SPF in T showed no difference with P2,both of them were significant higer than in P5 and N （F=34.99 P〈0.01）; （3） The PI in colorectal carcinoma（T）were significantly higher than P2, P5 and N,The PI in P2 were significantly higher than N （F=31.9696 P〈0.05）, （4）the aneuploidy in distal colorectal carcinoma were significantly higher than that in proximal colon （P〈0.001）. Conclusion： （1）The preliferative activity in colorectal carcinoma was significantly higher than the tissue surrounding it.（2）The proliferation behavior in P2 is still very active,so P2 is not the lowest safe limitation for surgical resection. （3）There exist difference in biological behavior between the proximal and distal colorectal carcinoma, comparaly,distal colon is more invasive.