胞浆抗原CyCD79a在B细胞型急性淋巴细胞白血病的表达及其意义(附83例分析)

Expression of cytoplasmic antigen CyCD79a and it's significance for B-cell acute lymphoblastic leukemia:Analysis of 83 cases

目的检测急性白血病(AL)患者胞浆CyCD79a,探讨其在协助诊断B细胞型急性淋巴细胞白血病(B-ALL)中的作用。方法采用直接免疫荧光法对134例急性髓细胞白血病(AML)及31例T细胞型急性淋巴细胞白血病(T-ALL)患者骨髓或外周血进行胞浆内抗原CyCD79a标记染色,83例B-ALL患者同时进行胞浆内抗原CyCD79a和CyCD22标记染色,以CD45/SSC设门法进行三色流式细胞术分析。结果134例AML及31例T-ALL患者CyCD79a均阴性;83例B-ALL中,CyCD79a阳性率占98.8%,而CyCD22阳性率仅占59%。结论CyCD79a是B系列的特异性标志,在协助诊断B-ALL时,敏感性高于CyCD22。

Objective To evaluate the role of CyCD79a in diagnosing B-ALL. Methods The cytoplasmic antigen Cy- CD79a was detected in 134 patients with AML, 31 patients with T-ALL and both CyCD79a and CyCD22 were detected in 83 patients with B-ALL by flow cytometry. Results The results of CyCD79a were negative both in patients with AML and patients with T-All, CyCD79a was positive in 82 （98.8%） among 83 patients with B-ALL, the positive rate of CyCD22 was only 59%. Conclusion The positive expression rate of CyCD79a is higher than that of CyCD22, which will benefit the diagnosis of B-cell acute lymphoblastic leukemia.