双歧杆菌微胶囊的制备和稳定性研究

Preparation of Microencapsulation of Bifidobacterium and Study on its Stability

本研究以明胶、黄原胶、果胶等为复合胶体,先用胶类物质包裹菌体形成耐酸的保护层,再用海藻酸钠和壳聚糖交联形成耐酸性能更好的双层微胶囊。使用正交设计优化工艺条件,试验制得的微胶囊在人工胃液中处理3h后存活率75%,活菌数为4.1×1010(cfu/g)。在直接把湿润的微胶囊加入酸奶中,15天后最高活菌数2.3×1010cfu/g,存活率56%。为双歧杆菌微胶囊产品的开发和利用提供一种新的方法。

Our study used hydrocolloids such as gelatine, xanthan glue and pectin as an inner layer in the microencapsulation. The outer layer was made from chitosan-alginate sodium by using superficial cross linking technology. Optimize operation condition use orthogonal design. The survival rate ofmicroencapsulated bifidobacterium is 75% after deal with in simulated gastrointestinal conditions 3 hours and living bifidobacterium is 4.1 ×10^10 （cfu/g） . The survival rate of microencapsulated b ifidobacterium is 56% and living bifidobacterium is 2.3×10^10 （cfu/g） after 15 days storage in yoghourt.