鱼腥草注射液治疗呼吸道合胞病毒感染的体外实验研究

Effects of Houttuynia Herb Injection on RSV Infection in Vitro

目的探讨鱼腥草注射液体外抗呼吸道合胞病毒(RSV)的有效性。方法采用MTT比色法检测鱼腥草对Hep2单层细胞的毒性作用;以利巴韦林为对照,通过空斑试验和试管观察细胞病变法确定鱼腥草对RSV的抑制作用。结果鱼腥草最大稀释度(1∶10)对细胞无毒性,不影响细胞形态及生长;鱼腥草(1∶10)和利巴韦林(5 mg/L)均可以完全抑制稀释度为10-5(100TCID50)的RSV、半数抑制10-4(1 000 TCID50)的RSV,二者均可使空斑明显变小。结论鱼腥草注射液对RSV有体外抑制作用,对培养细胞无毒性。

Objective To investigate the safety and the efficacy of Houttuynia Herb on RSV infection in vitro. Methods To determine the toxicity of Houttuynia Herb injection on Hep2 monolayer cells with MTT colorimetry. The inhibiting effects of Houttuynia Herb injection on RSV infected Hep2 cells were observed by Plaque forming unit（PFU） assay and the method of CPE in cuvette. The Ribavirin was used as control. Results The maximal concentration（1 ： 10） of Houttuynia was safe to Hep2 cells and did not affect the configuration and development of Hep2 cells. Both Houttuynia（ 1 ： 10） and Ribavirin 5 mg/L could inhibit 100TCID50 RSV completely and inhibit 1 000TCID50 RSV in half. The effects of two drugs could also make the viral plaques smaller or reduced obviously. Conclusion Houttuynia Herb injection is safe for culture cells and can inhibit RSV which infected Hep2 monolayer cells in vitro.