结直肠癌MMP7和MMP13的表达和意义

Study of the expression and significance of MMP7 and MMP13 in colorectal adenocarcinoma

目的探讨MMP7和MMP13在结直肠腺癌中表达及意义。方法用免疫组化PV9000法检测61例结直肠腺癌和61例癌旁结直肠黏膜组织MMP7和MMP13表达情况。结果61例结直肠腺癌和61例癌旁结直肠黏膜组织MMP13阳性率分别为95.08%和39.62%,腺癌组织MMP7和MMP13表达强度显著强于癌旁结直肠黏膜组织(P<0.01);MMP7在结直肠腺癌中的表达强度与组织学分级、浸润深度、临床病理分期、淋巴道转移有关(P<0.05),与发病部位及远处转移无关(P>0.05);MMP13的表达强度与TNM分期、淋巴道转移及术后5年内死亡有关(P<0.05),与发病部位、组织学分级、浸润深度、远处转移及术后复发转移无关(P>0.05)。结论MMP7和MMP13在结直肠腺癌的发生及淋巴道转移中可能起重要作用,测定MMP7和MMP13有助于判断其预后,MMP7和MMP13强阳性者预后差。

Objective To study of the expression and significance of MMP7 and MMP13 in colorectal ad Methods The expression of MMP7 and MMP13 was examined by PV9000 immunohistochemical technique in 61 cases of colorectal adenocardnomas and 61 cases of colorectal tissues near to carcinomas. Results The positive rates of MMP13 was 95.0896 in colorectal adenocarcinomas and 39.62 % in colorectal tissues near to carcinomas , the MMP7 and MMP13 expression was stronger in which with colorectal adenocarcinomas than in which with colorectal tissues near to carcinoma（ P 〈 0.01 ）. Of the colorectal adenocarcinomas , the positive intensities of MMP7 was associated with the histological grade and clinicopathological stage as well as lymph node metastasis diseases . Of the colorectal adenocarcinornas, the expression of MMP13 was associated with the lymph node metastasis diseases and poor survival（P〈 0.05）, but not correlated with the tumour position, histological grade, tumour depth and distant metastasis disease（P 〉 0.05）, Conchtsion MMP7 and MMP13 may be play an important role on the development and lymph node metastasis of colorectal adenocarcinomas. It is an useful marker for predicting the prognosis of colorectal adenocarcinomas.