妊娠中期因子在口腔癌中的表达及其意义

Expression and Significance of Midkine in Oral Carcinoma.

目的:研究妊娠中期因子(m idk ine,MK)在口腔癌中的表达及其意义。方法:应用免疫组织化学、W estern免疫印迹方法,对50例人口腔癌组织、30例良性肿瘤组织、30例正常口腔组织进行了MK蛋白表达的研究。结果:阳性产物出现于癌细胞胞浆中,呈棕黄色颗粒,良性肿瘤组织有微弱表达,正常组织无表达或有微弱表达。口腔癌中MK的表达明显高于对照组(P<0.01)。分类及分期观察,MK蛋白表达量随着口腔癌分期级别的递增呈上升趋势,后期尤其是Ⅲ期和ⅣA期有淋巴结转移的病例表达量呈中强、强阳性者居多。免疫组织化学结果与W estern免疫印迹结果基本一致(P<0.05)。结论:MK蛋白在口腔癌组织中高表达,并与疾病的恶性度密切相关。

Objective： To study the expression and significance of midkine in oral carcinoma. Methods： Immunohistochemistry and Western -blotting analysis for MK were performed on 50 cases of oral carcinoma, 30 cases of benign lesion and 30 cases of normal tissue. Results ： Positive products （ - brown - yellow grains） lied in the cytoplasm of cancer cells, and in cell nucleus occasionally. Benign tumors expressed faintly and normal tissues didn＇t express or express faintly. The quantity of MK in cancer was higher than the control groups （ P 〈 0.01 ）. The expression of MK protein enhanced with progression of classification and stage, especially in most Ⅲ and Ⅳ A stage cases which had lymph nodes metastasis and expressed mid - strong, or strong positive. The result of immunohistochemistry was the same as the result of Western - blotting （ P 〈 0. 05 ）. Conclusion ： The protein of MK was overexpressed in oral carcinoma, and was correlated with malignancy degree of disease.