马铃薯再生体系的建立及其遗传分析

Establishment and Genetic Analysis of Regeneration System of Potato

以马铃薯品种中薯2号为试材,筛选不同的培养条件建立了较高效的马铃薯再生体系,并对其再生过程进行了蛋白质检测和RAPD分析。愈伤组织诱导的最佳培养基为MS+6-BA2.25mg.mL-1+NAA0.1￣0.2mg.mL-1,愈伤组织的诱导率达到73.17%～76.01%。自然生长、初代培养和继代培养的马铃薯再生体的水溶性蛋白之间存在着一些差异。利用RAPD标记技术,通过对54个引物的严格筛选,获得了3个扩增产物略有差异的引物。

The potato cuhivar Zhongshu No.2 was used as a plant material to establish the efficient regeneration system by choosing different mediums. The protein test and RAPD analysis were carried out during the tissue culture. The optimal medium for callus formation was MS ＋ 6- BA 2.25 mg·mL^-1 ＋ NAA 0.1 -0.2 mg·mL^-1, and the induction rate could reach to 73.17%-76.01%. There were some differences in water soluble proteins for the natural, primary cultured and subcultured potatoes. Three out of fifty-four primers used in the RAPD analysis could amplify polymorphic bands among the three different cultural states. The results suggest that there may be some variations during the establishment of the regeneration of potatoes.