旱柳Q106组织培养及快繁体系的建立

Tissue culture and rapid propagation of Salix matsudana Q106 in vitro

以旱柳(Salixmatsudana)Q106春季萌生嫩枝为外植体,建立了“以芽繁芽”的组织培养快繁体系。丛生芽培养基为MS+BA0.4mg/L+NAA0.2mg/L。继代培养基为MS+BA0.4mg/L+NAA0.2mg/L和BA0.4mg/L+NAA0.4mg/L;MS+BA0.4mg/L+NAA0.2mg/L和BA0.4mg/L+IBA0.2mg/L交替使用。最佳生根培养基为MS+GA30.2mg/L+NAA0.5mg/L。

Rapid propagation system of Salix matsudana Q106 was established by using one axillary bud stem as explants which derived from internodal sections of 1-year-old Salix matsudanaQl06. The results obtained as follows： the axillary bud can obtain better multiplication on MS ＋0.4 mg/L BA ＋0.2 mg/L NAA culture,while developed plants were sub-cultured on MS＋0.4 mg/L BA＋0.2 mg/L NAA and 0.4 mg/L BA＋ 0.4 mg/L NAA or MS＋0.4 mg/L BA＋0.2 mg/L NAA alternate with 0.4 mg/L BA＋0.2 mg/L IBA culture. The optimum culture for rooting is MS＋0.2 mg/L GAa ＋0.5 mg/L NAA.