吸烟大鼠肺组织病理学改变及与VEGF表达相关性研究

The relationship between histopathological changes and VEGF expression in lung tissues of the passive smoking rats

目的探讨被动吸烟大鼠肺组织病理变化及其与血管内皮生长因子(VEGF)表达的相关关系。方法以12周龄雌性SD大鼠为研究对象,采用被动吸烟法,取被动吸烟3、6、9周的大鼠肺组织进行病理学检查及VEGF免疫组织化学检测,将两者进行相关性分析。结果吸烟大鼠支气管肺组织内出现以淋巴细胞为主的炎症细胞浸润,纤毛脱落,杯状上皮细胞化生。吸烟6周时出现部分肺泡壁破裂,肺泡腔扩大。吸烟9周大鼠与同龄对照组大鼠比较,其平均肺泡数减少(P<0.05),肺泡内衬间隔增加(P<0.05),呈现轻度肺气肿样改变。VEGF表达下降与肺泡数减少呈正相关(P<0.05),与肺泡间隔数增加呈负相关(P<0.05)。结论被动吸烟大鼠肺组织病理变化符合肺气肿样改变,且与VEGF的变化呈一定的相关性。VEGF可能是吸烟致大鼠肺气肿发病过程的重要影响因素。

Objective To investigate the relationship between pathological changes and vascular endothelial growth factor （VEGF） expression in the lung tissues of passive smoking rats. Methods The model of passive smoking method in 12-week- old female SD rats was established. The lung tissue in 3, 6, 9 - week passive smoking rats were taken respectively and pathological examination and VEGF immunohistochemical measurement were carried out, and the relationship between the pathological changes and the VEGF expressing was analyzed. Results There were inflammatory cells infiltration and cilia desquarnating and goblet epithelium cells metaplasia in the lung tissues in smoking rats. There were alveolar wall breaking partly and alveolar space enlarging in 6 - week smoking rats. In 9 - week smoking rats, early emphysema - like changes appeared. The average alveoli pulmonum number dccreased and the alveolus inner lining interval increased. The deceased of VEGF expressing was in positive correlation with the decrease of alveoli pulmonum number, and in negative correlation with the increase of alveolar septum number. Conclusion The pathological changes of the lung tissues in passive smoking rats consistentcd with emphysema-like changes, VEGF is possibly an important factor in the pathology of emphysema caused by smoking.