丹参注射液对小鼠脾脏树突状细胞增殖的影响

Effect of Injection Salvia Miltiorrhiza Bunge on Proliferation of Dendritic cellsfrom mouse spleen

目的:观察丹参(Salvia Miltiorrhiza Bunge,SMB)注射液对小鼠脾脏树突状细胞(Dendrirtic cells,DC)的增殖作用。方法:采用MTT法,以细胞因子(GM-CSF+IL-4)作对照,观察不同浓度SMB以及细胞因子+不同浓度的SMB对小鼠脾脏DC的增殖作用。结果:SMB在试验浓度为100、500、1000μg/ml时可显著促进小鼠脾脏DC增殖。说明SMB单独使用时可明显刺激小鼠脾脏DC增殖。但其刺激强度均明显低于细胞因子组;SMB+细胞因子,与对照组相比均有显著的增殖作用,与单独使用细胞因子组相比,其低浓度组(50、100、500μg/ml)协同作用显著。结论:SMB不仅能直接促进小鼠脾脏DC的增殖,而且与细胞因子有显著的协同作用。

Objective： To investigate the effect of Injection Salvia Miltiorrhiza Bunge （SMB）on proliferation of Dendritic cells （DC） from mouse spleen. Methods. Compared with the cytokine（GM - CSF ＋ IL - 4）, to investigate the effect of SMB of different concentrations and cytokine ＋ SMB of different concentrations on proliferation of DC from mouse spleen by MTT. Results： SMB（ 100, 500,1000 μg/ ml ） could stimulate the proliferation of the DC significantly, but the stimulations weren＇t obvious compared with the cytokine. SMB ＋ cytokine, compared with the negative control, had obvious promoting effect and the lower concentrations （50,100, 500 μg/ml） better than the cytokine. These results above indicate SMB could not only stimulate the proliferation of DC but also significantly do in coordination with the cytokine. Conclusion： SMB may play the role by its growth factor-like function, and by cooperating with the cytokine in the regulation of DC.