气相色谱法测定线虫中的脂肪酸含量

Determination of Fatty Acids in Nematode by Gas Chromatography

气相色谱法测定线虫中的脂肪酸含量郑文晖，谭炳炎，汤丽芬，李章旺（中山医科大学中心实验室广州５１００８９）１前言线虫是一种微小的生物，采用传统的方法测定其脂肪酸需要大量的样品，这在培养过程中很难做到。本实验直接用试剂把线虫里的结合脂肪酸直接酯化出来，减...

ematode is a small organism and the traditional purification method needs a lot of nematodes , but it is difficult for us to foster many nematodes.The fatty acids in nematode were esterified directly in our analytical method,so that,the error is reduced the and sensitivity is improved. The results showed that thisimproved analysis method could be used for rapid and satisfactory determination of the fatty acid change during storagr of this group of nematodes.The fatty acids in the nematode were analysed by gas chromatography. Seven kinds of main saturatd and unsaturated fatty acids with 16 to 20 carbon atoms were separated on the FFAP capillary column.The results of quantitative and qualitative analysis are satisfactory.