用DNA阳性及阴性标本制备专用DNA marker的实验研究

Experimental study on making a new DNA marker from positive and negative DNA samples

目的:以α地中海贫血基因诊断所需的m arker为例,探讨一种制备新的DNA m arker的方法。方法:选取相应实验所能检测到的所有阳性标本及阴性标本1份,常规抽提DNA及PCR扩增,将所有标本的PCR产物混合即可制成该实验所需专用DNA m arker。结果:3份阳性标本扩增后分别获得2000bp、1800bp、1600bp、1300bp条带,分别对应-3α.7、正常、-4α.2、--SEA的位置,混匀后所得专用m arker包含以上条带。结论:新组合的DNA m arker定位清晰,方法简便、经济、易行。

Objective： To take the DNA marker in the gene analysis of a thalassemia tor example to explore a new way of making DNA marker in need. Methods ：Every kinds of the positive samples and a negative sample were collected. The DNA were abstracted and PCR amplication were performed in routine. The PCR productions of the positive and negative samples were mixed together and the DNA marker were made. Results,The bands of the new DNA marker were located clearly in the right position the experiment needed, Conclusion： The method is easy to operate and save money.