摘要
高效液相色谱法测定肿瘤患者血清中唾液酸的含量王金惠,李亢宗,魏晴霞,李志杰(兰州医学院兰州730000)(兰州炼油厂医院兰州730060)1前言唾液酸是C9糖酸的衍生物,它主要与蛋白质和脂质结合而广泛存在于细胞膜、内质网和线粒体等膜性细胞器中。唾液酸...
A method for the determination of sialic acid (SA) in serum with high performance liquid chromatography was established. The condition were Sheer C18 , 10μm , column, sulfuric acid solution (pH 3.0) eluent and UV-210 detection. The recovery and detection limit of SA were 99.42% and 0.003 nmol/L respectively. The calibrations curve was linear within the range of 0. 032-3.23 nmol/L. The SA in serum samples of 34 healthy subjects and 92 cancer patients were determined by this method. The results showed that the contents of SA in cancer patients were significantly higher than those of healthy ones. The positive rates of lung, stomach . esophagus, breast and uterus cervix cancers were 72.6%, 77.8%, 76.7%, 72.2% and 60.0% respectively.
出处
《色谱》
CAS
CSCD
北大核心
1996年第2期145-146,共2页
Chinese Journal of Chromatography
关键词
唾液酸
血清
肿瘤患者
高效液相色谱
high performance liquid chromatography, sialic acid, cancer patient's serum