高效液相色谱法测定肿瘤患者血清中唾液酸的含量

Determination of Sialic Acid in Serum of Cancer Patients by High Performance Liquid Chromatography

高效液相色谱法测定肿瘤患者血清中唾液酸的含量王金惠，李亢宗，魏晴霞，李志杰（兰州医学院兰州７３００００）（兰州炼油厂医院兰州７３００６０）１前言唾液酸是Ｃ９糖酸的衍生物，它主要与蛋白质和脂质结合而广泛存在于细胞膜、内质网和线粒体等膜性细胞器中。唾液酸...

A method for the determination of sialic acid (SA) in serum with high performance liquid chromatography was established. The condition were Sheer C18 , 10μm , column, sulfuric acid solution (pH 3.0) eluent and UV-210 detection. The recovery and detection limit of SA were 99.42% and 0.003 nmol/L respectively. The calibrations curve was linear within the range of 0. 032-3.23 nmol/L. The SA in serum samples of 34 healthy subjects and 92 cancer patients were determined by this method. The results showed that the contents of SA in cancer patients were significantly higher than those of healthy ones. The positive rates of lung, stomach . esophagus, breast and uterus cervix cancers were 72.6%, 77.8%, 76.7%, 72.2% and 60.0% respectively.