用反相高效液相色谱法纯化白细胞介素－2条件初探

Exploration on Conditions for Purification of Interleukin-2 by Reversed-Phase High Performance Liquid Chromatography

用反相高效液相色谱法纯化白细胞介素－２条件初探胡明（沈阳军区军事医学研究所沈阳１１００３１）关键词反相高效液相色谱法，白细胞介素－２１前言高效液相色谱法（ＨＰＬＣ）是一项分辨率很高的液相色谱技术，具有速度快，灵敏度高、样品可回收等优点。除被广泛地应用...

n this paper, some factors affecting the purification of interleukin-2 (IL-2) have been summarized, such as ion strength, gradient program and selection of flow rate etc. We can conclude that in reversed-phase high performance liquid chromatography, for sample in high salt concentration, the salt should be removed before the sample was injected into the column in order to avoid sedimentation in the column and pipeline. We also find that very small changes of elution gradient will strongly affect the results of separation. With different samples, we should find out the most suitable gradient program by repeated experiments. Too-high or too-low flow rate is unfavourable to the results of separation. In order to avoid air bubbles and equilibrate the column, while stopping gradient program, the method that concentration of organic solvent is cut down in reverse gradient program can be used.