反相高效液相色谱对8种噻唑类衍生物保留性能的研究

Study on the Retention Behavior of Eight Thiazole Derivatives by Reversed-Phase High Performance Liquid Chromatography

在ODS反相往上，考察了不同浓度下的甲醇／水对8种噻唑衍生物保留值的影响。并把不同浓度的冲洗剂所得保留值和关系式lnK'=a＋cCb中的参数a、c作线性关联，得到很好的相关性，同时考察了不同种类的无机盐和有机腹对噻唑类衍生物容量因子k'的影响。结果表明，甲醇：0．2mol/LHAc－NSAc缓冲液（PH4．0）：三乙胶=45：54:1（V/V）时，在UV254nm检测波长下，所有组分在25min内可实现较理想的分离。

The capacity factors k' of eight thiazole derivatives on reversed-phase ODS column with methanol/water as eluant were measured, the linear regression between the lnk'values and methanol concentrations according to equation lnk'=a+cCb was carried out and a good linear relationship was obtained. The parameters a and c with various concentrations of methanol as eluant were measured. ln addition, the effect of various salts and triethylamine on the capacity factors of thiazole derivatives were studied. A mobile phase of methanol: 0. 2 mol/L HAc-NaAc buffer solution (pH 4. 0): triethylamine=45: 54: 1 and UV detection at 254 nm brought about satisfactory results.