新基因XTP3反式激活基因TPA的克隆化研究

Cloning and Identification of Gene A Transactivated by Hepatitis B Virus XTP3 Protein

目的:应用抑制性消减杂交(SSH)技术构建乙型肝炎病毒(HBV)X蛋白(HBxAg)反式激活基因XTP3的差异表达的cDNA消减文库,克隆XTP3反式激活相关基因。方法:依据我室构建的HBV X蛋白反式激活基因XTP3差异表达的cDNA消减文库,利用生物信息学技术获得新基因XTP3TPA的编码序列,对其可能的氨基酸序列进行分析比较,并对其进行克隆化研究。结果:发现了HBV XTP3反式激活作用的新的靶基因,命名为XTP3TPA。结论:这一发现为阐明HBV XTP3蛋白的反式激活作用及其机制开辟了新的研究方向。

Objective： Cloning and identification of new gene-XTP3TPA transactivated by hepatitis B virus XTP3 protein, Methods： On the base of subtractive cDNA library of genes transactivated by XTP3 protein of hepatitis B virus, the coding sequence of new gene, named as XTP3TPA, was obtained by bioinformatics methods, Polymerase chain reaction （PCR） was conducted to amplify XTP3TPA gene, Results： The coding sequence of new gene was cloned and identification successfully. Conclusion.. A new gene has been recognized as the new target transactivated by HBV XTP3 protein, These results brought some new clues for studying the biological functions and pathogenesis of the viral proteins.