极谱免疫法测定人血清补体活性的研究

Determination of Complement Level in Serum by Polarographic Immunoassay

提出一种极谱免疫法测定人血清补体活性的新方法，利用补体作用下的免疫溶血反应释放出的具有拟过氧化物酶活性的血红蛋白，催化邻苯二胺和Ｈ_２Ｏ_２的反应，通过酶促产物２，２′－二氨基偶氮苯的极谱检测来确定补体的活性。测定范围为０．１０～１．００ＣＨ_（５０）ｕｎｉｔ／ｍＬ，检出限为０．０８ＣＨ_（５０）ｕｎｉｔ／ｍＬ，灵敏度比分光光度法高１０倍，一次可分析２５份血样，已用于正常人和病人血清中补体活性的测定。

A new method for determination of complement level in serum by polarographicimmunoassay was developed in this paper. This method is based on the reaction between ophenylenediamine and hydrogen peroxide catalyzed by hemoglobin from the sensitized cells incomplement-mediate hemolysis, producing an electroactive compound 2,2'-diaminoazobezen(DAA)and determining the DAA by linear-sweep polarography.The linear calibrationcurve is obtained in the range of 0. 10 to 1. 00 CH50 unit/mL and the limit of detection is 0. 08CH50 unit/mL. The sensitivity of the method is ten times more than that in the spectrophotometric method reported.And 25 samples at a time can be determined. The proposedmethod has been applied to the determination of complement level in healthy human serumand patient serum.