慢性乙肝患者α-干扰素治疗过程血清CHE活性检测分析

Changes of Serum Cholinesterase (CHE) Activity in Patients with Chronic B Hepatitis During α-Interferon Treatment

目的:观察α-干扰素治疗前后慢性乙肝患者胆碱酯酶(CHE)活性及乙肝病毒DNA(HBV-DNA)含量的变化。方法:CHE活性采用酶动力学方法,HBV-DNA采用实时荧光PCR定量法。结果:经α-干扰素治疗后,32例患者HBV-DNA含量明显下降(P<0.01),24例HBV-DNA含量无明显变化(P>0.05);所有经α-干扰素治疗后的56例患者CHE明显升高(P<0.01),而未经α-干扰素治疗的39例慢性乙肝患者与正常对照一样CHE无明显变化(P>0.05),血清CHE的升高与α-干扰素的抗病毒效果无相关性。结论:CHE的动态监测可反映α-干扰素治疗过程慢性乙肝患者肝纤维化的改善程度。

Objective To investigate the changes of serum cholinesterase （CEH） activity and HBV - DNA content in patients with chronic B hepatitis after α - interferon treatment. Methods Serum CHE activity （with bio - chemical method） and HBV - DNA content （with RT -PCR） were determined in 56 patients with chronic B hepatitis both before and after six months α -interferon treat- ment as well as in 39 patients with chronic B hepatitis without ot - intenferon treatment and 51 controls. Results In all the 56 patients treated with α - interferon, the serum CHE activity readings were significantly higher than those before treatment （ P 〈 0.01 ）. In the 39 patients without interferon treatment and 51 controls, the CHE activity readings changed little throughout the observation period. However only 32 of the 56 patients had their HBV - DNA contents dropped significantly （ vs before treatment, P 〈 0.01 ）. Contents of HBV - DNA dropped very little in the remaining 24 patients （ vs before treatment, P 〉 0.05 ）. The increase of CHE activity after α - interferon therapy was not correlated with the anti -viral efficacy. Gonclusion As generally accepted, the degree of hepatic fibrosis in patients with chronic hepatitis was inversely proportional to the CHE activity. Dynamic monitoring of serum CHE activity during α - interferon treatment might indirectly reveal the improvement of the status of hepatic fibrosis.