双抗原酶斑点-ELISA快速检测幽门螺杆菌抗体的研究

Study on Double Antigens Dot-ELISA for Rapid Detection of Helicobacter Pylori Antibodies

[目的]为胃幽门螺杆菌(H.p)感染的血清学诊断和流行病学调查,研究一种快速、简便、实用、特异的检测手段。[方法]在制备H.p可溶性抗原-辣根过氧化物酶(H.pAg-HRP)基础上,用硝酸纤维膜为载体建立双抗原酶斑点-ELISA试验;用本法与双抗原-ELISA、间接ELISA、市售ELISA试剂盒对成人人群血清标本进行对比。[结果]双抗原酶斑点-ELISA检测血清H.p抗体与市售ELISA相比,特异性为82.9%、敏感性为90.2%、符合率为87.0%,且与市售ELISA的检测结果差异无显著性。[结论]双抗原酶斑点-ELISA检测H.p抗体方法特异性、敏感性均达到检测要求,并具有快速、简便、实用特点,适合基层用于流行病学调查。

[objective] To develop rapid, simply, practicable and specific methods for sero-diagnosis and epidemiological survey on Helicobacter pylori （H. p） infection in human. [Methods] Double antigens dot-ELISA was established on the basis of conjugation of H. p＇s antigens with horseradish peroxidase and making the fiber membrane of nitric acid as the carrier. Double antigens dot-ELISA was used to detect H. p＇s antibodies of serum specimen and compared with the methods of double antigens ELISA, indirect ELISA and ELISA reagent box. [Results] The specificity of double antigens dot- ELISA was 82. 9% and the sensitivity was 90.2 %, and the agreement rate was 87.0%. And the detection result was similar to ELISA reagent box （P〉0. 05）. [Conclusion] Double antigens dot-ELISA method is not only specific and sensitive but also rapid, simply and practicable, and is suitable for epidemiological survey on H. p antibodies.