毛细管电泳－激光诱导荧光法分离检测DNA片段及基因扩增产物

Analysis of DNA Fragments and PCR Products from Oncogene Using Capillary Electrophoresis with Laser-Induced Fluorescence Detection

以羟丙甲基纤维素和非交联聚丙烯酰胺浴液为筛分介质，将毛细管电泳－激光诱导荧光法用于ＤＮＡ片段及基因扩增产物的分离检测．探讨了非胶筛分介质中高分子化合物的浓度、电解质的浓度、内插试剂用量等对ＤＮＡ片段分离检测的影响；考察了ＤＮＡ片段迁移时间和峰面积的重现性及ＤＮＡ片段定量检测的关系．建立了一种快速、灵敏的ＤＮＡ片段及基因扩增产物分离检测方法．

Capillary electrophoresis-laser induced fluorescence detection system is applied to detect DNA fragments and PCR products from oncogene using hydroxypropylmethylcellulose and no-cross-linked polyacrylamide as sieving media. The effects of sieving medium concentration,electrolyte concentration and intercalating agent concentration on separation of DNA fragments have been investigated in detail. The relationship between peak area and concentration of DNA fragments in pressure injection has been compared with that of electrokinetic injection.